Scientist II
Job Description
Scientist II
Location: Worcester, MA
Work Arrangement: 100% onsite
Contract Duration: 12 months
Overview
We are seeking a highly motivated and talented Scientist II to join our In Vivo Antibody Discovery and Molecular Core team in Worcester, MA. This is a newly created role driven by increased workload, supporting a team of six scientists.
This R&D-focused position centers on high-throughput next generation sequencing (NGS), primarily working with human cells. The role involves upstream processing, including working with primary cells (p cells), DNA extraction, sequencing preparation, and data analysis, with results shared with downstream scientists.
Key Responsibilities
Experimental Design & Execution
- Independently design, execute, and interpret experiments utilizing microfluidics to amplify heavy and light chain genes and generate DNA libraries for deep sequencing of single B cells captured in droplet emulsions
- Understand project objectives and adapt experimental plans as needed while meeting timelines
Molecular Techniques
- Perform RNA isolation and DNA purification
- Amplify antibody heavy- and light-chain variable regions from single cells or lysates
- Prepare and submit DNA libraries for next generation sequencing (MiSeq/MiSeq i100)
Bioinformatics & Data Analysis
- Conduct sequencing data analysis of cells
- Perform in silico antibody genetic analysis and data visualization using standard and custom bioinformatics tools
- Utilize coding/data analysis at a basic to intermediate level (preferred)
Collaboration
- Present and discuss experimental findings with cross-functional teams
- Support multiple programs across the in vivo antibody discovery portfolio
- Work both independently and collaboratively while managing multiple projects
Qualifications
- 3–5 years of experience
- Hands-on experience with DNA sequencing (Illumina) – required
- Microfluidic experience – required
- Experience working with next generation sequencing (NGS)
- Molecular biology expertise
- DNA/RNA purification experience
- Primary cell (p cell) experience
- Data analysis background – preferred
- Basic to intermediate coding/data experience – preferred
Education
- Bachelor’s or Master’s degree required
- PhD is overqualified (OQ)
Required Skills & Experience
- Master’s degree in biology, immunology, cell/molecular biology, or related discipline with experience in single B cell sequencing research and 2+ years of relevant experience (MS)
- Demonstrated hands-on expertise in droplet microfluidics of single B cells and next generation sequencing of IgG variable heavy and light chains
- Familiarity with next generation sequencing platforms (Illumina MiSeq and MiSeq i100) and B cell genetic data analysis software/databases (FLASH, IgBlast, Usearch, IMGT)
- Experience in reverse transcription, RNA/DNA isolation and purification, overlap extension PCR, primer design, cDNA library generation, and gel electrophoresis
- Protein and cell detection methods experience (ELISA and/or FACS) – preferred
Additional Details
- Reason for Need: New role due to increased workload
- Team Size: 6