Jobs in Leicester, MA

100% ONSITE

Pay: $48-$52/hr

REQUIRED:

• Minimum: Master’s degree + 2‑5 years relevant experience
• Bachelor’s acceptable with 5 years strong hands‑on background
• Background in microbiology, immunology, cell/molecular biology, or biotech
• Hands‑on experience with NGS (Illumina MiSeq/iSeq) – must‑have
• Microfluidics: emulsion, droplet generation, custom or commercial systems
• B‑cell purification and handling
• Molecular biology: polymerase reactions, RNA/DNA purification, indexing
• Basic data analysis; familiarity with commercial sequencing software
• Scripting in Python or R for data processing (plus)
• Bioinformatics tools (IMGT, IgBLAST)
• Protein detection methods (ELISA, similar assays)
• Experience with other NGS platforms (Thermo Fisher, PacBio, ONT) – nice to have

Job Description:

B-Cell Sequencing is the focus of this group - downstream (Extracting antibodies from individual B cells and sequencing them to find new drug candidates)

The successful candidate will support the development of new paired antibody sequencing technologies from single B cells using droplet microfluidics. Responsibilities include experimental planning and execution, comprehensive B cells sequencing analysis and presentation, and detailed documentation to support multiple programs across our in vivo antibody discovery portfolio.

Responsibilities

- Experimental Design & Execution: Independently design, execute and interpret experiments utilizing microfluidics to amplify heavy and light chain genes and generate DNA libraries for deep sequencing of single B cells captured in droplet emulsions.

- Understand the broad objectives of the project as well as their role in achieving those objectives, and modify the experimental plan when required. Set and meet experimental timelines.

- Molecular Techniques: Demonstrate a high level of proficiency in conducting RNA isolation, and DNA purification, and related methods to amplify antibody heavy- and light-chain variable regions from single cells or lysates and submit DNA libraries for next generation sequencing (MiSeq/MiSeq i100).

- Bioinformatics Data Analysis: Perform comprehensive in silico antibody genetic analysis and data visualization using standard and custom antibody bioinformatic tools – to guide program decisions and deliver biotherapeutic candidates.

- Cross-Functional Collaboration: Collaborate with multidisciplinary teams to present/discuss experimental findings to ensure alignment with overall biotherapeutic development goals.

- Ability to work independently and as part of a team, managing multiple projects simultaneously.